Fig. 5

A2M regulates the stem cell characteristics of CD9⁺ cells. a The interactions of CD9 and A2M in the network were analyzed by GeneMANIA using the parameters limited to physical interactions, genetic interactions, and pathways to score nodes and source organism Homo sapiens as additional parameters. b Protein expression of A2M and EGR1 in CD9⁺ cells after A2M knockdown. c CD9 expression after A2M knockdown. (THP-1: sh-Control = 78.66%, sh-A2M 1 = 53.2%, sh-A2M 2 = 51.89%; HL-60: sh-Control = 58.62, sh-A2M 1 = 34.3%, sh-A2M 2 = 31.6%). d The proliferation ability of CD9⁺ cells after A2M knockdown. Error bars represent ±SD of triplicates. e, f The drug-resistant ability and migration ability of CD9⁺ cells after A2M knockdown. Error bars represent ±SD of triplicates. g Schematic summary of the role of A2M in regulating the stemness of CD9⁺ AML-LSCs. *p < 0.05, **p < 0.01, ***p < 0.001