Fig. 8

The schematic diagram that doxorubicin cytotoxicity induced by the remarkably differentially expressed miRNA and the protective mechanism of extracellular vesicles from hP-MSCs. The cytotoxicity of doxorubicin for hUC-MSCs is severe. The treated hUC-MSCs showed great discrepancy compared to normal hUC-MSCs, which was supported by the results of miRNA sequencing. Among the differentially expressed miRNA, some were remarkably labeled, the target gene prediction of which indicated that the adverse effects, cell cycle arrest, inflammatory infiltration, ROS production, calcium deposit, and mitochondrial dysfunction, may be involved the cytotoxicity of doxorubicin and result in apoptosis. In this process, the extracellular vesicles from hP-MSC can exert protective effect through the downregulation of SCOTIN mediated by hsa-miR-11401 and reduce the expression of cleaved-caspase 3, alleviating the apoptosis induced by doxorubicin