Fig. 4

Ischemia-hypoxia leads to EPC accumulation and CXCL12 increase in the corpus callosum of neonatal rat. a, b The accumulation of EPCs and claudin5-positive vessels in the corpus callosum were observed by immunofluorescence, and the number of EPCs and the density of vessels were quantified. Scale bar = 200 μm in a and 50 μm in b. c CXCL12 protein level of the corpus callosum was examined by western blotting. Data were analyzed using one-way ANOVA and shown as mean ± SD (n = 4, triplicates per group). *P < 0.05, **P < 0.01, **P < 0.001 vs. Ctrl group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. P3+1 group; ^ΔP < 0.05 vs. P3+4 group. Data were analyzed using Student’s t test