Fig. 5

Transplantation of EPCs promoted oligovascular remodeling in WMI rat. a Immunofluorescent staining showed EPC-derived CD31-positive ECs (green) integrated into the host brain and formed vascular-like structure, scale bar = 5 μm. The IOD of CD31 was quantified. b HI-induced OPC apoptosis was detected by PDGFRα immunofluorescent and TUNEL staining, scale bar = 20 μm. The percent of TUNEL and PDGFRα double-positive cells was analyzed. c MBP immunofluorescent staining and quantitative analysis were conducted for myelination evaluation, scale bar = 20 μm. d Representative electron microscopic images of the corpus callosum were shown. The g-ratio was calculated in each group. All values are expressed as the mean ± SD (n = 4 for each group). *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham group; ^#P < 0.05, ^##P < 0.01 vs. vehicle group; ^ΔP < 0.05 vs. EC-pEPCs group. Data were analyzed using Student’s t test