Fig. 7
a, b Relative mRNA and protein level of PTEN in control, vector, and PTEN-OE groups. c Protein levels of α-SMA in control, CIH, CIH+EXO, and CIH+EXO-301a groups as measured by Western blotting. d, e Apoptosis rate in four groups as determined by flow cytometry. f RT-qPCR results of PTEN in four groups (***p < 0.001). g Protein level of PTEN, HIF-1α, LC3I/II, and p62 as assessed by Western blotting. h–k mRFP-GFP-LC3 staining and quantitative results of autophagosomes, autolysosomes, and autophagic vacuoles in the four groups. l, m Relative mRNA and protein level of TLR4 in control, vector, and TLR4-OE groups. n, o Level of apoptosis in control, CIH, CIH+EXO, and CIH+EXO-301a groups. p–r Protein levels of α-SMA, TLR4, and p65 as measured by Western blotting. Data are expressed as mean ± SD (n = 6; **p < 0.01, ***p < 0.001)