Fig. 4

KAT6A regulated stemness of OBMSCs through Nrf2/ARE signaling pathway. a Forty-eight hours later, the expressions of Nrf2, GCLC, and NQO1 in YBMSCs that transfected with scrambled siRNA or Nrf2 siRNA were explored by qRT-PCR (n = 3). b The ROS level in YBMSCs that transfected with scrambled siRNA or Nrf2 siRNA was explored (n = 3). c CCK-8 was performed to explore the proliferative capacity of YBMSCs that transfected with scrambled siRNA or Nrf2 siRNA (n = 3). d The colony-forming abilities of YBMSCs that transfected with scrambled siRNA or Nrf2 siRNA were explored by crystal violet after culture for 12 days (n = 3). e After osteogenic induction for 14 days, expressions of osteogenic-related genes of Runx2, OCN, and BMP2 were detected in YBMSCs that transfected with scrambled siRNA or Nrf2 siRNA (n = 3). f After osteogenic induction for 21 days, alizarin red staining was performed in YBMSCs that transfected with scrambled siRNA or Nrf2 siRNA (n = 3). g After overexpressing KAT6A in OBMSCs in the content of Nrf2 siRNA, the ROS level was explored (n = 3). h After overexpressing KAT6A in OBMSCs in the content of Nrf2 siRNA, CCK-8 was performed to explore the proliferative capacity (n = 3). i After overexpressing KAT6A in OBMSCs in the content of Nrf2 siRNA, the colony-forming abilities were explored by crystal violet after culture for 12 days (n = 3). j After overexpressing KAT6A in OBMSCs in the content of Nrf2 siRNA, expressions of osteogenic-related genes of Runx2, OCN, and BMP2 were detected (n = 3). k After overexpressing KAT6A in OBMSCs in the content of Nrf2 siRNA, alizarin red staining was performed (n = 3). Scr-Vec, scramble + vector; Scr-OeKAT6A, scramble + OeKAT6A; SN-OeKAT6A, SiNrf2 + OeKAT6A; a “OBMSCs + Scr-Vec” compared with “OBMSCs + Scr-OeKAT6A”; b “OBMSCs + Scr-OeKAT6A” compared with “OBMSCs + SN-OeKAT6A”