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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells

Fig. 2

Akt inhibition prevents increases in tenomodulin, while Smad3 inhibition appears to accelerate tenomodulin production. ac Quantified western blot band densitometry showing tenomodulin is not detectable following 7 d of treatment with MK-2206 + TGFβ2, indicating tenogenesis is disrupted when Akt signaling is inhibited. d Representative western blot showing cells treated with MK-2206 + TGFβ2 do not produce tenomodulin at 7 d. e, f MSCs treated with SIS3 + TGFβ2 produce more tenomodulin than cells treated with TGFβ2 alone at 7 d. g Representative western blot showing MSCs production of tenomodulin increases with 7 d of TGFβ2 and SIS3 treatment, compared to controls, and MSCs treated with TGFβ2 or SIS3 alone. *p < 0.05, **p < 0.01. Bars = mean ± standard deviation

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