Fig. 2From: Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cellsAkt inhibition prevents increases in tenomodulin, while Smad3 inhibition appears to accelerate tenomodulin production. a–c Quantified western blot band densitometry showing tenomodulin is not detectable following 7 d of treatment with MK-2206 + TGFβ2, indicating tenogenesis is disrupted when Akt signaling is inhibited. d Representative western blot showing cells treated with MK-2206 + TGFβ2 do not produce tenomodulin at 7 d. e, f MSCs treated with SIS3 + TGFβ2 produce more tenomodulin than cells treated with TGFβ2 alone at 7 d. g Representative western blot showing MSCs production of tenomodulin increases with 7 d of TGFβ2 and SIS3 treatment, compared to controls, and MSCs treated with TGFβ2 or SIS3 alone. *p < 0.05, **p < 0.01. Bars = mean ± standard deviationBack to article page