Fig. 3
From: Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells
Akt inhibition impacts tenogenic cell morphology. Representative images (20×) of MSCs treated with TGFβ2 and MK-2206. Actin cytoskeleton (green) and cell nuclei (blue) are shown in MSCs at 24 h, and 3, 7, and 14 d (a–p). d, h, l, p MSCs treated with both TGFβ2 and the Akt inhibitor, MK-2206, had disruptions in cell morphology and did not appear elongated or fibroblastic, compared to b, f, j, n cells treated with TGFβ2 only and a, e, i, m controls. c, g, k, o MK-2206 alone did not appear to drastically impact cell morphology. Scale bar = 100 μm