Fig. 5
From: Akt signaling is activated by TGFβ2 and impacts tenogenic induction of mesenchymal stem cells
Smad3 inhibition does not alter tenogenic cell morphology. Representative images (20×) of TGFβ2 and SIS3-treated MSCs. a–h Actin cytoskeleton (green) and cell nuclei (blue) are shown in MSCs at 24 h, 3 d, and 7 d. d, h, l MSCs treated with both TGFβ2 and the Smad3 inhibitor, SIS3, appeared more fibroblastic and elongated, compared to a, e, i controls and b, f, j cells treated with TGFβ2 only. c, g, k SIS3 alone did not appear to impact cell morphology. Scale bar = 100 μm