Fig. 4

Conversion of interscapular brown adipose tissue (iBAT) to white adipose tissue (WAT) in angiotensin II (Ang II)-induced hypertensive mice. We induced hypertension in C57BL/6 (wild-type (WT)) mice (infused with Ang II at a rate of 750 ng/kg per minute for 28 days (AngII group) or 0.9% NaCl (control group)). a Noninvasive tail cuff monitoring of the systolic blood pressure (SBP) of the above 2 groups of mice was performed. Two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001 versus the corresponding control; ns indicates not significant. b Representative HE staining of aortic sections showing the aortic intima and media thickness (n = 4). c Representative HE staining of iBAT sections showing the conversion of BAT to WAT in iBAT from the above 2 groups of mice. Immunoblotting analysis of UCP-1 and PGC-1α protein expression in Ang II-stimulated brown adipocytes either differentiated from S-MSCs (d) or isolated from the iBAT of mice (e)