Fig. 5

Brown adipose tissue deficiency enhanced angiotensin II (Ang II)-induced hypertension and target organ remodeling. We induced hypertension (infused with Ang II at a rate of 750 ng/kg per minute or 0.9% NaCl for 28 days) in C57BL/6 mice and established 4 groups: the WT-C group (infused with 0.9% NaCl), -BAT-34-C group (iBAT-deficient mice infused with 0.9% NaCl), WT-Ang II group (infused with Ang II), and -BAT-34-Ang II group (iBAT-deficient mice infused with Ang II). a Noninvasive tail cuff monitoring of the systolic blood pressure (SBP) of the above 4 groups of mice (n = 5–6) was performed. Student’s t test. b, c Representative HE staining of aortic sections and quantification of the aortic intima and media thickness (n = 4). One-way ANOVA. d Representative HE staining of heart sections (fibrotic tissues are indicated by arrows) (n = 4). All data that were analyzed by Student’s t test or one-way ANOVA passed the normality test. *P < 0.05, **P < 0.01, ***P < 0.001 versus the corresponding control; ns indicates not significant