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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Novel live cell fluorescent probe for human-induced pluripotent stem cells highlights early reprogramming population

Fig. 6

a Heatmap showing 386 differentially expressed genes (between BDL-E5+ and BDL-E5 cells) after RNA sequencing of four cell types (DiPS, DPSC, BDL-E5+, and BDL-E5 cells) after FACS at 7 dpn of DPSC2 upon reprogramming (n = 2). b Venn diagram showing the number of genes up-regulated in BDL-E5+ and BDL-E5 cells, as obtained from RNA sequencing data. A total of 386 genes were differentially expressed significantly between the two cell types. c Table showing the relevant genes that were differentially expressed among DiPS, DPSC, BDL-E5+, and BDL-E5 cells. d Graphs showing mRNA expression of CREB1 (i) and PRKAB2 (ii) obtained by qPCR from RNA isolated from DiPS, DPSC, BDL-E5+, and BDL-E5 cells of DPSC2 obtained after FACS at 7 dpn (n = 3). *p < 0.05 denotes significance compared with DiPS; ^p < 0.05 and ^^^p < 0.001 denote significance compared with BDL-E5+

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