Fig. 1

Generation of muscle progenitor cells (MPC) from human iPSC using small molecules. a Schematic outline of the generation of MPC from human iPSC using a combination of CHIR99021 and givinostat or CHIR99021 only. b Morphology of differentiating cells from 3 human iPSC lines (CF-iPSC, DF-iPSC-1, and DF-iPSC-2) at 7 days. Bar = 200 μm. c Morphology of replated MPC and differentiated MPC in muscle differentiation medium from 4 human iPSC lines at day 14. Bar = 200 μm. Characterization of givinostat-induced MPC: d The treated hiPSC at day14 expressed Pax7 and desmin. Bar = 100 μm. e The differentiated myotubes expressed MF20 as shown by immunostaining. Bar = 100 μm. The white arrows point at the multinuclear cells in fused cells