Fig. 2

Givi-MPC exhibited superior proliferation and migration capacity. a Representative images and b quantitative estimate of cell migration by adult human myoblasts and control-MPC, Givi-MPC (arrow). Cells were stained with Calcein AM (green). Bar = 1 mm. b Quantitative estimate of migrated cells. Givi MPC showed the highest number of cells migrated as compared with human myoblasts (P < 0.0001) or CHIR99021 induced MPC (P < 0.0001). No significant difference was observed between human myoblasts and control-MPC. c Heat map of the human RT2 motility PCR array. d Differentially expressed genes related to migration in Givi-MPC vs. control-MPC using human cell motility PCR array. e Proliferation curves of human myoblasts vs. MPC using CCK-8 assay. *P < 0.05; ^#P < 0.05 vs. control-MPC. n = 6. f Morphology of MPC colonies. Bar = 500 μm. Number of colonies (g) and percentage of colonies with different cell number in color (h). control-MPC: CHIR99021-induced MPC; Givi-MPC: CHIR99021 and givinostat-induced MPC