Fig. 5

Givi-MPC decreased muscle necrosis and fibrosis in Mdx/SCID mice 1 M after CTX injury. a Representative images of HE and Masson's trichrome staining in Mdx/SCID mice after transplantation with human myoblasts or control MPC or Givi-MPC 1 M after CTX injury. Bar = 500 μm (× 4) and bar = 100 μm (× 20). Quantification of necrotic muscle fibers after treatment with human myoblasts (b), control MPC (c), and Givi-MPC (d) 1 M after CTX injury. e Comparison of muscle necrosis among human myoblasts or control-MPC- or Givi-MPC-transplanted Mdx/SCID mice. f Representative images of tissue stained with Sirius red after transplantation with MPC. Bar = 500 μm (× 4) and bar = 100 μm (× 20). The boxed area is shown below at high magnification. Quantification of muscle fiber fibrosis in TA muscle treated with human myoblasts (g), control-MPC (h), and Givi-MPC (i) 1 M after CTX injury. j Muscle fibrosis after transplantation of different MPC. n = 6 in the human myoblasts and control MPC groups and n = 11 in the Givi-MPC group