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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Local anesthetics impair the growth and self-renewal of glioblastoma stem cells by inhibiting ZDHHC15-mediated GP130 palmitoylation

Fig. 4

Identity of palmitoylated proteins mediated by ZDHHC15. a Principles of the ABE capture methods. U251 GSC was dissolved and incubated with ZDHHC15 antibody. In addition, it was cleaved with palmitate in the HAM+ group. The HAM− condition was used as a negative control. After the ABE reaction, streptavidin beads were used to enrich biotinylated proteins. The proteins enriched under HAM+ and HAM− conditions were identified by mass spectrometry (MS). In the HAM+ sample, proteins with at least 2-fold higher abundance compared to the control were considered as candidate proteins. Probability: 0–19% (2–3 times; n = 37), 20–49% (3–4 times; n = 6), and > 50% (> 4 times; n = 31). b Venn diagram showing the relationship between the expression patterns of different DHHCs in glioma using the Human Protein Atlas (HPA), previously validated S-acylated proteins, predicted S-acylated proteins using the CSS-Palm version 4.0 software, and function previously reported in glioma. c Lysates from U251 GSCs were subjected to IP with the ZDHHC15 antibody, followed by immunoblotting (IB) with anti-GP130, anti-LRP12, and anti-RIF1 antibodies

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