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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Human fetal liver MSCs are more effective than adult bone marrow MSCs for their immunosuppressive, immunomodulatory, and Foxp3+ T reg induction capacity

Fig. 1

FL-MSCs share several common characteristics with adult BM-MSCs and reveal a greater ex vivo expansion ability than BM-MSCs. FL and BM-MSCs cultured between passage 3 and 6 were characterized by their cell morphology, their cell surface marker expression, and their differentiation and proliferative capacity. a After 14 days of culture, both FL (passage 3) and BM (passage 3)-MSCs showed a spindle-shaped fibroblast-like morphology representative of MSCS (original magnification × 4). Pictures are representative of 3 independent experiments (n = 3). b Flow cytometry analysis showing relative expression of MSC (CD271, CD166, CD146, CD90, CD106, CD105, CD73, CD54, CD51, CD44, CD29), hematopoietic (CD45, CD14, and CD34), and MHC (HLA-DR and HLA ABC) markers in FL (passage 3) and BM (passage 3)-MSCs. The dark gray histograms represent specific antibodies and light gray histograms represent corresponding isotype controls. Data are representative of 3 independent experiments (n = 6). c Comparison of proliferation of FL and BM-MSCs by MTS assay over a period of 8 days. Data are represented as mean value ± SEM from 3 independent experiments (n = 9). Unpaired Student t test was performed to generate P values. ns, non-significant; D, day. *P < .05, **P < .01, ***P < .001, ****P < .0001

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