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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human fetal liver MSCs are more effective than adult bone marrow MSCs for their immunosuppressive, immunomodulatory, and Foxp3+ T reg induction capacity

Fig. 2

FL-MSCs are more immunosuppressive than BM-MSCs. CFSE-labeled, CD3/CD28-activated CD3+CD25 effector T cells were co-cultured with FL-MSCs or BM-MSCs in 6 different MSC to T cell ratios. After 3 days, proliferation of CD4+ (a) and CD8+ T cells (b) was measured by flow cytometry based on CFSE dilution. Each histogram bar represents the percent of dividing cells. The first bar represents the unstimulated T cells alone (n = 6), the second bar represents the CD3/CD28-stimulated T cells alone (n = 6). Further bars depict T cells co-cultured with either BM-MSCs in red (n = 6) or FL-MSCs in blue (n = 6). All data are collected from 2 different experiments. Data are represented as mean value ± SEM. One-way ANOVA analysis was performed to generate P values. ns, non-significant; *P < .05, **P < .01, ***P < .001, ****P < .0001. c A flow cytometry representative of proliferation assay at 1:6 MSC to T cell ratio. T cells co-cultured with BM-MSCs are depicted in red and their FL counterparts are depicted in blue. Each histogram bar represents the percent of dividing cells. Beads, anti-CD3 and anti-CD28 activation beads; TCs, T cells; T convs, conventional T cells

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