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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Human fetal liver MSCs are more effective than adult bone marrow MSCs for their immunosuppressive, immunomodulatory, and Foxp3+ T reg induction capacity

Fig. 6

CD4+T regs induced from FL-MSCs are more immunosuppressive than those induced from BM-MSCs. Activated CD3+CD25 effector T cells were co-cultured with BM- or FL-MSCs in a fixed 1:5 MSC to T cell ratio. After 4 days, CD4+CD25+ iT regs were selected and co-cultured with CFSE-labeled, CD3/CD28-activated CD3+CD25 responder T cells for another 3 days. Then, the proliferation of CD4+ (a) and CD8+ (b) T cells was measured by flow cytometry based on CFSE dilution. Each histogram bar represents the percent of dividing cells. The first white bar represents the unstimulated T cells group (n = 6), while the second black bar represents the activated T cells group (n = 6). T cells co-cultured with BM-MSCs iT regs are depicted in red (n = 6) and their FL counterparts in blue (n = 6). c Representative flow cytometry histogram of proliferation assay at 1:5 MSC to T cell ratio. Non-stimulated T cells are depicted in light gray and CD3/CD28 stimulated T cells are in dark gray. T cells co-cultured with BM-MSCs iT regs are depicted in red and their FL counterparts are depicted in blue. Each histogram bar represents the percent of dividing cells. Data are represented as mean value ± SEM collected from 2 different experiments. One-way ANOVA analysis was performed to generate P values. ns, non-significant; TCs, T cells; iT regs, induced regulatory T cells; T convs, conventional T cells; TCs, T cells; iT regs, induced regulatory T cells; Beads, anti-CD3 and anti-CD28 activation beads. *P < .05, **P < .01, ***P < .001, ****P < .0001

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