Fig. 1

Intrinsic levels of CD10 positively correspond to lipid accumulation. a Representative merged images of AdipoRed staining of lipids (in green) and Hoechst 33342 staining of nuclei (in blue) across differentiated ASCs from 12 subjects (between passage 6 and 8) are shown. The scale bar represents 100 μm. b CD10 levels at D0 (basal undifferentiated) and at D12 (differentiated) of ASCs from the 12 subjects are shown based on qRT-PCR analysis. Fold expression changes of CD10 are compared to the S8. Each value is the mean ± SEM from three independent wells for qRT-PCR of each population performed in triplicates. c The LDs are quantified in the differentiated ASCs of the 12 subjects by using a MATLAB algorithm as a read-out for adipogenesis levels. Each value is the mean ± SEM (n=6). d Correlation of CD10 levels at D0 and lipids at D12 post-differentiation are shown. CD10 levels are highly correlated with the lipids accumulated upon differentiation (Correlation coefficient R = 0.9962). e The relative mRNA expression levels of CD10 by qRT-PCR in CD10 knockdown (KD), CD10 overexpression (OE) compared with their respective controls C (KD) and C (OE) ASCs are shown normalized to RPL27. Fold expression changes are compared to the C (KD) level. Each value is the mean ± SEM from three independent replicates. f Western blot analysis shows the protein expression of CD10 in CD10 KD and CD10 OE ASCs compared to the respective controls. GAPDH was used as the loading control. g Representative merged images of AdipoRed staining of lipids (in green) and Hoechst 33342 staining of nuclei (in blue) of CD10 KD, CD10 OE and the control ASCs lines are shown. The scale bar represents 100 μm. h Quantification of the lipid content was done by using a MATLAB algorithm. CD10 OE and CD10 KD ASC populations showed respective increase and decrease in lipids compared to the controls. Each value is the mean ± SEM for each population performed in five-independent batches. All statistical analysis was performed using Student’s paired t test, ***p < 0.001, **p < 0.01, *p < 0.05