Fig. 2
High CD10 expressing ASCs show increased browning capacity. a Relative mRNA levels of UCP1 are quantified before and after FSK treatment by qRT-PCR normalized to RPL27. Fold expression changes are compared to the subject S8 without FSK treatment. Each value is the mean ± SEM from three independent replicates. b Relative mRNA levels of CD10 are quantified at D0 and D12 by qRT-PCR normalized to RPL27. Fold expression changes are compared to the subject S102 (D0). Each value is the mean ± SEM from three independent replicates. c The LDs are quantified by using MATLAB analysis before and after FSK treatment. d Correlation of CD10 levels with UCP1 expression are shown with the Pearson coefficient R2 value. Good correlation is observed between UCP1 induction and CD10 levels (R = 0.8610). e Correlation between intrinsic CD10 levels with reduction in LDs following browning are shown with the Pearson coefficient R2 value. f Representative merged images of AdipoRed staining of lipids (in green) and Hoechst 33342 staining of nuclei (in blue) in CD10 knockdown (KD) and overexpression (OE) cells with controls with (out) FSK treatment. The scale bar represents 100 μm. g The LDs are quantified by using MATLAB analysis as described in Experimental Procedures. The values are normalized to CD10 OE ASCs. Each value is the mean ± SEM from three independent replicates. h Relative mRNA levels of browning marker gene UCP1 are quantified by qRT-PCR normalized to RPL27. Fold expression changes are compared to CD10 OE cells before FSK treatment. Each value is the mean ± SEM from three independent replicates. All statistical significance was assessed by using Student’s paired t-test: ***p < 0.001, **p < 0.01, *p < 0.05