Fig. 3

CD10 inhibits endogenous lipolysis resulting in higher lipid accumulation in mature ASCs. Western blot analysis shows detection of a phosphorylated form of HSL and d ATGL using antibodies against phospho-HSL (P-S660), total HSL, phospho-ATGL (P-S406), and total ATGL in CD10 KD, OE, and control cells. Quantification of HSL phosphorylation are shown in b CD10 KD and c CD10 OE cells. Similar phosphorylation levels are shown for ATGL in e CD10 KD and f CD10 OE cells. CD10-mediated alteration on mitochondrial function expressed as Oxygen consumption rate (OCR; in pMoles/min) are shown in mature ASCs g before or h after FSK treatment. Western blot analysis showing the i expression of COXIV and its relative quantification between j CD10 KD and k OE cells compared to the controls. For all the blots, GAPDH was used as internal control, and statistical analysis was performed using Student’s paired t test (n = 3), ***p < 0.001, *p < 0.05, ns denotes non-significant, p > 0.05