Fig. 3From: Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in miceAnalysis of surviving hPBMCs in the injected right retina and contralateral left retina by flow cytometry and real-time PCR. a–c Flow cytometry results showing surviving hPBMCs in retinal cell suspensions prepared a 1 month after transplantation and b 3 months after transplantation. The PE fluorescence (red) is from CM-DiI and the FITC fluorescence (green) is from immunostaining using a human mitochondrial antibody. Zone Q2 is the double-labeled area indicative of transplanted hPBMCs. c Statistical analysis of surviving hPBMCs in right and left retinas at 1 and 3 months after transplantation. d RNA expression of the human mitochondrial marker gene (indicative of hPBMCs). Expression level is shown relative to that at 1 month post-injection. P < 0 .05 by paired sample t testsBack to article page