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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Migration of pre-induced human peripheral blood mononuclear cells from the transplanted to contralateral eye in mice

Fig. 3

Analysis of surviving hPBMCs in the injected right retina and contralateral left retina by flow cytometry and real-time PCR. ac Flow cytometry results showing surviving hPBMCs in retinal cell suspensions prepared a 1 month after transplantation and b 3 months after transplantation. The PE fluorescence (red) is from CM-DiI and the FITC fluorescence (green) is from immunostaining using a human mitochondrial antibody. Zone Q2 is the double-labeled area indicative of transplanted hPBMCs. c Statistical analysis of surviving hPBMCs in right and left retinas at 1 and 3 months after transplantation. d RNA expression of the human mitochondrial marker gene (indicative of hPBMCs). Expression level is shown relative to that at 1 month post-injection. P < 0 .05 by paired sample t tests

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