Fig. 8

Comparison of the angiogenic and osteogenic differentiation potential of F- and C-SVFs (n = 5/group). Double-positive staining for uptake of acLDLs and binding of lectin extracted from U. europaeus was observed in the F- and C-SVF groups (a) (bar, 50 mm), without significant difference between the two groups (b). Based on the assessment of the tube length, both the F- and C-SVF groups had high potential (c) (bar, 100 mm) with no significant difference between the groups (n = 5/group) (d). Both groups were positive for ALP and alizarin red staining (e) with no significant differences of ALP activity (f) and alizarin red mineralization (g) between the F- and C-SVF groups (n = 5/group). NS, not significant. F-SVF, freshly isolated stromal vascular fraction; C-SVF, cryopreserved stromal vascular fraction