Fig. 2

MiR-34a mediates MSC dysfunction under hyperglycaemic conditions. MSCs were cultured with normal or high glucose medium for 28 days with or without transfection with miR-34a mimic, NC or miR-34a inhibitor for 48 h, respectively. a QRT-PCR analysis was applied to detect the expression of miR-34a. b Cell viability was determined by CCK8 assay. c Expression of VEGF and bFGF were measured by ELISA. d Cellular senescence was analysed by SA-β-gal staining. e The senescence related protein of p21and p16 were determined by western blot. Each column represents mean ± SD from three independent experiments. *P < 0.05 vs. NG group. ^#P < 0.05 vs. HG group