Fig. 4

MiR-34a induces cellular dysfunction under hyperglycaemia of MSCs by regulating SIRT1-FoxO3a autophagy dynamics. MSCs were cultured with normal or high glucose medium for 28 days with or without transfection with miR-34a inhibitor or siRNA-SIRT1 for 48 h, respectively. a The protein expression were determined by western blot. b Cellular senescence was analysed by SA-β-gal staining. c Expression of VEGF and bFGF were measured by ELISA. Each column represents mean ± SD from three independent experiments. *P < 0.05 vs. NG, ^#P < 0.05 vs. miR-34a I group