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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1+/−−deficient mouse

Fig. 2

Complementation of Neurog1-deficiency is distinct from general chimerism. a, A’ High magnification of the basal turn in a chimeric Neurog1+/+cochlea does not show the engraftment of iPSCs in the spiral ganglia, (solid outline) compared to bD’ examples from two separate chimeric and complemented Neurog1+/− cochleas in which iPSCs extensively contribute to the SGN (dotted outline). Chimerism was seen in both the e Neurog1+/+ brain and f the Neurog1+/− brain, indicating that while both embryos successfully formed chimeras, donor cells did not specifically contribute to the SGN in the absence of a vacant niche. (Images are from a single Neurog1+/+ wild type (1/2), sample number 111807 and two separate complemented heterozygotes (2/3) sample numbers 111812 and 111810). All scale bars are 100 μm. Scale bar in A’ corresponds to aB’, scale bar in f corresponds to e. DAPI 4,6-diamidino-2-phenylindole, TUJ1 class III beta-tubulin antibody, GFP green fluorescent protein antibody against endogenous fluorescence, MYO6 unconventional myosin VI antibody

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