Fig. 3

Expression of selected and highlighted EPC-regulated markers in healthy donors’ PBMCs by single-cell RNA sequencing. a Cluster identification inside circulating population of 33,000 PBMCs from healthy donor analyzed by single-cell sequencing with Seurat software. b–e Quantification by single-cell RNA sequencing of molecular markers in healthy donor PBMCs: background of cells with negative expression is colored in gold and positive cells for the markers appeared in dark blue. b Expression of endothelial-related markers selected by literature curating. c Expression of markers selected by literature curating and were found to have lympho/myeloid expression. d Expression of markers selected by literature curating and were found to have an expression in monocytes either in CD16+ subpopulation or in CD14+ subpopulation. e Expression of highlighted markers that were found to be regulated previously between endothelial populations of different tissues