Fig. 2

CircTTC3 promotes OGD-induced astrocyte injury in vitro. a The astrocytes were treated with OGD and the expression of circTTC3 was measured by qPCR in the cells. b–g The OGD-treated astrocytes were treated with control shRNA or circTTC3 shRNA. b The cell apoptosis was assessed by flow cytometry analysis in the cells. c The expression levels of Bax, Bcl-2, caspase-3, cleaved caspase-3, and β-actin were determined by Western blot analysis in the cells. The results of Western blot analysis were quantified by the ImageJ software. d The levels of LDH in the culture medium were assessed by colorimetric assays in the cells. e The levels of TNF-α and IL-1β were analyzed by ELISA assays. f Senescence was evaluated by MUG assays. g The protein expression of H2AX, γH2AX, and β-actin was determined by Western blot analysis in the cells. The results of Western blot analysis were quantified by the ImageJ software. Data are presented as mean ± SD. Statistic significant differences were indicated: *P < 0.05, **P < 0.01