Fig. 6

CircTTC3 regulates OGD-induced astrocyte injury and NSCs by the miR-372-3p/TLR4 axis. a, b The OGD-treated astrocytes were treated with control shRNA or circTTC3 shRNA or co-treated with circTTC3 shRNA and miR-372-3p inhibitor or pcDNA-TLR4. a The cell apoptosis was assessed by flow cytometry analysis in the cells. b The levels of LDH in the culture medium were assessed by colorimetric assays in the cells. c, d The NSCs were treated with control shRNA or circTTC3 shRNA or co-treated with circTTC3 shRNA and miR-372-3p inhibitor or pcDNA-TLR4. c The cell viability was measured by MTT assays in the cells. d The protein β-tubulin III expression were tested by qPCR and Western blot analysis, respectively. Data are presented as mean ± SD. Statistic significant differences were indicated: **P < 0.01