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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Endometrial regeneration with endometrial epithelium: homologous orchestration with endometrial stroma as a feeder

Fig. 2

Culture of endometrial epithelial cells with mouse embryonic fibroblasts. a, b Microscopic appearance of endometrial epithelial cells without feeder cells (a) and with MEF (b) in serial passage. Black bar is 500 μm. ce Cumulative area of colonies (c), colony formation (number) (d), and area of colonies (e) of endometrial epithelial cells in serial passages. Error bar indicates SEM. An asterisk means P < 0.05. ns means “not significant”. f Population doubling levels of endometrial epithelial cells when culture with MEF (red) and without feeder cells (blue). We could propagate endometrial epithelial cells with MEF for 111 days. Error bar indicates SEM. Dotted line indicated the observation period until the culture was terminated. g Immunohistochemical staining for endometrial epithelial cells and MEF at passage 4. Endometrial epithelial cells kept positive for pan-cytokeratin in serial passage. MEF expressed vimentin. Endometrial epithelial cells did not express vimentin. Nuclei were stained with DAPI. Yellow bar is 500 μm. Each experiment was done in triplicate. Abbreviation: MEF, mouse embryonic fibroblasts; SEM, standard error of the mean

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