Fetal liver hematopoiesis: from development to delivery

Table 1 Description of cytokines and growth factors produced in vivo that may be required for fetal liver HSC culture in the presence of hepatic cells. STM septum transversum mesenchyme, HSPC hematopoietic stem and progenitor cells

Cytokine/growth factor	Cell type promoted	Cell of origin in vivo	In vitro concentration ranges
SCF	HSPC	Endothelial cells	1–100 ng/ml	[22, 23, 25, 29, 48]
TPO	HSPC, Megakaryocytes	Endothelial/hepatocytes	1–100 ng/ml	[23, 48]
IGF2	HSPC	Hepatocytes	20 ng/ml	[22, 23, 55, 56]
ANGPTL2,3	HSPC	Hepatocytes	100 ng/ml	[22, 23]
BMP4	Hepatocyte	Cardiac mesoderm/STM	20 ng/ml	[41, 57]
FGF2	Hepatocyte	Cardiac mesoderm/STM	10 ng/ml	[41, 57]
HGF	Hepatocyte	Mesenchyme	10–20 ng/ml	[41, 42]
Oncostatin M	Hepatocyte	Macrophages, Stellate cells	10–20 ng/ml	[41, 43]

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