Fig. 2

Overexpressing miR-335-3p repressed NSC differentiation but maintained NSC self-renewal. NSCs were treated with NC-mimic, miR-335-3p mimic, NC-inhibitor, or miR-335-3p inhibitor. a Expression of miR-335-3p in NSCs detected by RT-qPCR. b Microscopic observation of neurosphere formation ability (day 7) (× 200). c Quantitative analysis for number and size of neurospheres. d Cell cycle distribution of NSCs measured by flow cytometry. e Western blot analysis for the expression of Fas and FasL. f Expression of self-renewal-related and differentiation-related genes assayed by RT-qPCR. g Representative protein bands and corresponding histograms of the expression of self-renewal-related and differentiation-related genes tested by Western blot analysis. The measurement data were described as mean ± standard deviation. Data comparisons among multiple groups were conducted by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05 versus NC-mimic or NC-inhibitor. The experiment was conducted three times