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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1

Fig. 4

NSC self-renewal was promoted by FoxM1-activated miR-335-3p. NSCs were treated with oe-NC, oe-FoxM1, si-NC, si-FoxM1, oe-FoxM1 + NC-inhibitor, and oe-FoxM1 + miR-335-3p inhibitor. a miR-335-3p expression in NSCs detected by RT-qPCR. b Statistical analysis of the number and size of neurospheres. c NSC cell cycle measured by flow cytometry. d Expression of self-renewal-related and differentiation-related genes in NSCs examined by RT-qPCR. The measurement data were expressed as mean ± standard deviation. Data analyses among multiple groups were performed by one-way ANOVA, followed by Tukey’s post hoc test. *p < 0.05 versus oe-NC or si-NC or oe-FoxM1 + NC-inhibitor. #p < 0.05 versus oe-FoxM1 + NC-inhibitor and oe-NC. The experiment was performed three times

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Stem Cell Research & Therapy

ISSN: 1757-6512