Fig. 1

Enhanced expression of CXCR4 and IL10 in human Ad-MSCs transfected with specific mRNAs. a Analysis of CXCR4 mRNA levels at different times after transfection with CXCR4-mRNA and CXCR4-IL10-mRNA. b Flow cytometry analysis of CXCR4 in WT and mRNA-transfected Ad-MSCs at different times post-transfection. c Immunofluorescence staining of CXCR4 in WT and mRNA-transfected MSCs 4 h after transfection with the different mRNAs (bar = 20 μm). Inserts with higher magnifications (red frame) indicate that CXCR4-expressing MSCs resulted in the protrusion of long filopodia at the cell periphery. Bar = 10 μm. d CXCR4 expression analyzed by western blot 4 h after transfection with the different mRNAs using vinculin as a control. e Analysis of mRNA IL10 levels analyzed at different time points after transfection with IL10-mRNA and CXCR4-IL10-mRNAs. f IL10 secretion measured by ELISA in WT and mRNA-transfected Ad-MSCs at different time points after transfection. Bars in the plots represent mean ± SD from three independent experiments, and statistical differences were analyzed by Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; versus WT-MSCs