Fig. 2
mRNA-transfected Ad-MSCs maintain the characteristic immunophenotype and secretome of WT-MSCs. a Immunophenotype analysis of WT-MSCs and mRNA-transfected Ad-MSCs. Representative analyses of characteristic MSC markers are shown. b Analysis of secreted PGE2, TGFβ1, IL8, IL6, IFNγ, and TNFα 4 h after transfection of Ad-MSCs with the different mRNAs. Levels of PGE2, IL8, and TGFβ1 were quantified by ELISA, while IL6, IFNγ, and TNFα levels were determined flow cytometry using fluorescence-encoded beads. Bars represent the mean ± SD of n = 3 different experiments