Fig. 3

Increased in vitro migration and immunosuppression ability of Ad-MSCs transfected with CXCR4 and IL10 mRNAs. a Representative images of transwell-migrating WT and mRNA-transfected Ad-MSCs (4 h post-transfection) in response to human (left) or murine (right) SDF-1. Cells in the transwell membranes were stained with hematoxylin (bar = 100 μm). b Quantification of the number of migrated cells in response to human (graphic above) or murine (graphic bellow) SDF-1. Data were collected from three randomly chosen fields under a microscope. c Inhibition of lymphocyte proliferation in vitro. Human PBMCs were cultured with or without WT or mRNA-transfected MSCs in the presence of PHA for 3 days at MSC/PMBC ratios of 1/10. The proliferation of CFSE-labeled PBMCs was assessed by flow cytometry, and the effect of Ad-MSCs on PBMCs was calculated as percentage of proliferation compared with the proliferative response in PHA-stimulated lymphocytes without Ad-MSCs (positive control, white bar). Data represents the mean ± SD of at least three different experiments analyzed by Tukey’s multiple comparison test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001