Fig. 2

Propofol impairs the differentiation of retinal neurons and glia. Immunohistochemical analysis of markers (green) of neuronal and glial differentiation at 3 d postfertilization (dpf) (a, c) and 2 dpf (b). DNA, blue. a, b Although the retinas of propofol-treated embryos (5 μg/ml propofol) contain an occasional ganglion cell (a, arrowheads), optic axon tracts are absent (b). L, lens. The dotted line indicates the area of the eye. Arrows indicated optic axon tracts. c Ganglion cells (zn-8), Müller cells (GFAP), and red/green cones (zpr-1). With the exception of Müller cells (arrowheads), all late-differentiated cell types are absent. n = 5. Scale bar, 75 μm