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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Mesenchymal stem cells modified by FGF21 and GLP1 ameliorate lipid metabolism while reducing blood glucose in type 2 diabetic mice

Fig. 2

Transduction of MSCs with lentiviral vector particles. a Expression of EGFP in MSCs transduced at an MOI of 40 under fluorescence microscopy. Bars = 100 μm. b Flow cytometry analysis of phenotype characterization of MSC-FGF21+GLP1. The phenotypes of CD73, CD90, CD105, CD34, and CD45 expression by MSCs were detected by flow cytometry. The green lines indicate the fluorescence intensity of cells stained with the corresponding antibodies, and the red lines represent isotype-matched negative control cells. c Analysis of EGFP fluorescence by flow cytometry at 48 h after transduction at different MOIs. The MOI ranges from 0 to 55, at intervals of 5. d MSCs transduced with FGF21+GLP1 lentivirus could differentiate into osteoblasts and adipocytes. Osteogenesis was examined by alizarin red S staining for mineral nodule deposition. Adipogenesis was observed by the presence of lipid vesicles and confirmed by oil red O staining. The picture in the red box shows the enlarged observation of lipid droplets in the cell. Bars = 100 μm

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