Fig. 7

Signaling pathways by which MSC-FGF21+GLP1 regulates lipid metabolism. Western blot analysis verified the signaling pathway by which MSC-FGF21+GLP1 cells regulate lipid metabolism. β-actin was the reference protein for cytoplasmic proteins, and histone 3 was the reference protein for nuclear proteins. p-AMPK, p-ACC, and p-HSL represent the levels of phosphorylation of these proteins. Cytoplasmic proteins were extracted to detect AMPK, ACC, FAS, and HSL. Nuclear proteins were extracted to detect SREBP1 and SREBP2. AMPK, adenosine monophosphate-activated protein kinase; SREBP, sterol regulatory element-binding proteins; ACC, acetyl-coenzyme A carboxylase; FAS, fatty acid synthase; HSL, hormone sensitive lipase. a Western blot analysis of the expression of the above genes in HepG2 cells, *P < 0.05, compared to Con. b Western blot analysis of the expression of the above genes in the liver, *P < 0.05, compared to Con