Table 1 Primer sequences used in RT-PCR
From: mTOR inhibitor INK128 promotes wound healing by regulating MDSCs
Gene | Forward primers | Reverse primers |
|---|---|---|
GAPDH | AGGTCGGTGTGAACGGATTTG | GGGGTCGTTGATGGCAACA |
p47phox | ACACCTTCATTCGCCATATTGC | CCTGCCACTTAACCAGGAACA |
gp91phox | AGTGCGTGTTGCTCGACAA | GCGGTGTGCAGTGCTATCAT |
Arg-1 | CTCCAAGCCAAAGTCCTTAGAG | GGAGCTGTCATTAGGGACATCA |
iNOS | GTTCTCAGCCCAACAATACAAGA | GTGGACGGGTCGATGTCAC |
IL-6 | CTGCAAGAGACTTCCATCCAG | AGTGGTATAGACAGGTCTGTTGG |
CD206 | CTCTGTTCAGCTATTGGACGC | TGGCACTCCCAAACATAATTTGA |
IL-10 | CTTACTGACTGGCATGAGGATCA | GCAGCTCTAGGAGCATGTGG |
IGF-1 | CACATCATGTCGTCTTCACACC | GGAAGCAACACTCATCCACAATG |
IL-1β | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
S100A8 | AAATCACCATGCCCTCTACAAG | CCCACTTTTATCACCATCGCAA |
S100A9 | GCACAGTTGGCAACCTTTATG | TGATTGTCCTGGTTTGTGTCC |