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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion

Fig. 3

Regulation of EMF on osteogenic differentiation of BMSCs. a Images of ALP and Sirius Red staining of BMSCs after culturing for 1 week; images of Alizarin Red staining of BMSCs after culturing for 2 weeks. b Semi-quantitative analysis of ALP, Sirius Red, and Alizarin Red staining among both groups (n = 6). Data are shown as mean ± SD. c Effects of EMF on osteogenic protein expression of BMSCs after culturing for 1 week (COL1, OCN, and BMP2) detected by western blotting. d Quantification analysis of the osteogenic protein expression (COL1, OCN, and BMP2) (n = 3). Data are shown as mean ± SD. e Effects of EMF on osteogenic gene expression of BMSCs after culturing for 4 days (ALP, OPN, and BMP2) detected by RT-qPCR (n = 3). *p < 0.05 compared to the Ctrl group, **p < 0.01 compared to the Ctrl group

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Stem Cell Research & Therapy

ISSN: 1757-6512