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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion

Fig. 5

Effects of the conditioned medium of BMSCs on undifferentiated BMSCs. a Immunofluorescence labeling for OPN of BMSCs cultured with the conditioned medium for 1 week. b Immunofluorescence labeling for Runx2 of BMSCs cultured with the conditioned medium for 1 week. c Fluorescence quantitative analysis of OPN and Runx2 (n = 5). d Gene expression of COL1, OPN, and Runx2 of BMSCs cultured with the conditioned medium was detected by RT-qPCR (n = 3). *p < 0.05 compared to the Blank group, **p < 0.01 compared to the Blank group, #p < 0.05 compared to the Ctrl group, ##p < 0.01 compared to the Ctrl group

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