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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Myogenic progenitor cell transplantation for muscle regeneration following hindlimb ischemia and reperfusion

Fig. 1

Cell characterization. a Immunocytochemical staining of MPC and NMC demonstrating positive desmin expression in MPC (red color) and negative desmin expression in NMC. Nuclear counterstaining performed by hematoxylin. b Live-cell imaging of Syto24 stained TdTomato MPCs, cultivated in skeletal muscle differentiation medium for 4 days (blue arrows: multinucleated myotubes). c Quantification of AChE activity per 2 × 105 cells in either MPC or NMC both cultivated in skeletal muscle differentiation medium. d Flow cytometric analysis of CD9 and CD98 surface marker in MPC from at least two different mice demonstrated as histogram (red). Isotype control stained cells (white histograms) used to determine threshold for positivity. AChE, acetylcholinesterase; MPC, myogenic progenitor cell; NMC, non-myogenic cells

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