Fig. 8

Assessment of muscle regeneration after warm ischemic injury. In order to quantify muscle generation, histological slides were analyzed and graded by a blinded histologist according to a 4-tier scale (0 = no signs of regeneration, 1 = mild signs of regeneration, 2 = moderate signs of regeneration, 3 = severe signs of regeneration). a Fourteen days after ischemic injury with 2h of WIT, both sham (C1) and cell injected (C2) animals displayed similar signs of regeneration. b In animals with ischemic injury (D1) and MPC-injection, a significantly higher muscle regeneration score was seen on POD 75 compared to animals without ischemic injury and MPC-injection. For intergroup comparison, the Mann-Whitney test was used. *p < 0.05; MPC, myogenic progenitor cell; POD, postoperative day; WIT, warm ischemic time