Fig. 9

Histological analysis POD 75. H&E staining of the tibialis anterior muscle 75 days after intramuscular injection of MPC in a uninjured muscle and b muscle subjected to 2h WIT. While no cellular aggregates were found in animals after ischemic injury (b), dense cell piles were seen in uninjured muscle samples (a, bottom left). In animals subjected to 2h WIT centrally located nuclei and thus signs of regeneration (b, bottom left, black circles) were still present on POD 75. H&E, hematoxylin and eosin; MPC, myogenic progenitor cell; WIT, warm ischemic time