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Table 1 Overview experimental groups

From: Myogenic progenitor cell transplantation for muscle regeneration following hindlimb ischemia and reperfusion

Experimental groups
Subgroups WIT Cell amount Cell type Endpoint Number
Baseline testing: MPC implantation and tracking was established (SHO-PrkdcscidHrhr mice)
  None 0.5 mio. cells Luci_GFP POD 35 n = 2
  None 1 mio. cells Luci_GFP POD 35 n = 2
  None 1 mio. cells—1 needle Luci_GFP POD 71 n = 4
  None 1 mio. cells—4 needles Luci_GFP POD 71 n = 4
  None 1 mio. cells—4 needles TdTomato POD 70 n = 2
Group A: Characterization of warm ischemic injury in a model of murine limb ischemia (C57BL/6 mice)
A1 30 min None POD 3, 7, 14 n = 3
A2 1 h None POD 3, 7, 14 n = 3
A3 2 h None POD 3, 7, 14 n = 3
A4 3 h None POD 3, 7, 14 n = 4
Group B: Myogenic progenitor cells and ischemia/reperfusion injury (C57BL/6 mice)
B1 2 h None Puffer POD 2 n = 8
B2 2 h 1 mio. cells—4 needles TdTomato POD 2 n = 5
Group C: Myogenic progenitor cells and muscle regeneration (C57BL/6 mice)
C1 2 h None Puffer POD 14 n = 8
C2 2 h 1 mio. cells—4 needles TdTomato POD 14 n = 5
Group D: Ischemic injury and cell engraftment (C57BL/6 mice)
D1 2 h 1 mio. cells—4 needles Luci_GFP POD 75 n = 7
D2 None 1 mio. cells—4 needles Luci_GFP POD 75 n = 8
  1. WIT warm ischemic time, POD postoperative day