Table 1 Overview experimental groups
Experimental groups | |||||
|---|---|---|---|---|---|
Subgroups | WIT | Cell amount | Cell type | Endpoint | Number |
Baseline testing: MPC implantation and tracking was established (SHO-PrkdcscidHrhr mice) | |||||
None | 0.5 mio. cells | Luci_GFP | POD 35 | n = 2 | |
None | 1 mio. cells | Luci_GFP | POD 35 | n = 2 | |
None | 1 mio. cells—1 needle | Luci_GFP | POD 71 | n = 4 | |
None | 1 mio. cells—4 needles | Luci_GFP | POD 71 | n = 4 | |
None | 1 mio. cells—4 needles | TdTomato | POD 70 | n = 2 | |
Group A: Characterization of warm ischemic injury in a model of murine limb ischemia (C57BL/6 mice) | |||||
A1 | 30 min | None | – | POD 3, 7, 14 | n = 3 |
A2 | 1 h | None | – | POD 3, 7, 14 | n = 3 |
A3 | 2 h | None | – | POD 3, 7, 14 | n = 3 |
A4 | 3 h | None | – | POD 3, 7, 14 | n = 4 |
Group B: Myogenic progenitor cells and ischemia/reperfusion injury (C57BL/6 mice) | |||||
B1 | 2 h | None | Puffer | POD 2 | n = 8 |
B2 | 2 h | 1 mio. cells—4 needles | TdTomato | POD 2 | n = 5 |
Group C: Myogenic progenitor cells and muscle regeneration (C57BL/6 mice) | |||||
C1 | 2 h | None | Puffer | POD 14 | n = 8 |
C2 | 2 h | 1 mio. cells—4 needles | TdTomato | POD 14 | n = 5 |
Group D: Ischemic injury and cell engraftment (C57BL/6 mice) | |||||
D1 | 2 h | 1 mio. cells—4 needles | Luci_GFP | POD 75 | n = 7 |
D2 | None | 1 mio. cells—4 needles | Luci_GFP | POD 75 | n = 8 |