Fig. 10

Catalase expression and activities during extended DPSC culture with or without exogenous H₂O₂ (50–200 μM) treatment. a qRT-PCR analysis of catalase gene expression by high proliferative (10–25PDs and 40–65PDs) and low proliferative (2–10PDs) DPSC sub-populations. Relative fold changes in enzymic antioxidant gene expression (RQ) were calculated using the 2^–ΔΔCt method, normalized versus an 18S rRNA housekeeping gene. b Total catalase activities for high proliferative (at 10–25PDs and 40–65PDs) and low proliferative (at 2–10PDs) DPSC sub-populations, treated as above. N = 3, values in the graphs represent mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001