Fig. 12

GSTZ1 gene expression and protein levels during extended DPSC culture with or without exogenous H₂O₂ (50–200 μM) treatment. a qRT-PCR analysis of GSTZ1 gene expression by high proliferative (10–25PDs and 40–65PDs) and low proliferative (2–10PDs) DPSC sub-populations. Relative fold changes in enzymic antioxidant gene expression (RQ) were calculated using the 2^–ΔΔCt method, normalized versus an 18S rRNA housekeeping gene. b Representative Western blot images and corresponding densitometric analysis of GSTZ1 protein levels by high proliferative (at 10–25PDs and 40–65PDs) and low proliferative (at 2–10PDs) DPSC sub-populations. For all Western blots, images from one representative experiment of three are shown. Densitometry data was normalized versus β-actin loading controls, with values subsequently normalized versus untreated high proliferative DPSCs at early PDs (10–25PDs). A.U. = Arbitrary units. N = 3, values in the graphs represent mean ± SEM, ***p < 0.001