Fig. 6

Oxidative DNA damage detection during extended DPSC culture with or without exogenous H₂O₂ (50–200 μM) treatment. Representative FITC (green, i–iv) and Hoechst nuclear stain (blue, v-viii) fluorescence microscopy images of 8-OHdG detection by immunocytochemistry, for a high proliferative sub-population, A1 (2–10PDs), b low proliferative sub-population, D4 (2–10PDs), and c high proliferative sub-population, A1 (45–60PDs). N = 3, scale bar 100 μm, × 200 magnification